Mitochondrial+ blend dosage and evidence
Written by Aaron CuhaReviewed Sep 2026
The short answer
Mitochondrial+ blend is a 55 mg vial of three peptides, sold at 9 mg SS-31, 16 mg TB-500 and 30 mg MOTS-c.
Evidence grade for Mitochondrial+ blend: Preliminary. The rule: studied in people, but no published study has had people take it. The sum on this page: 1 human study where nobody was given it. A grade counts how much has been done to people, not whether it works.
- People given it
- 0
- Human studies
- 0
- Found nothing
- 0
Dose
No human dose established
No published study on this page has given this compound to people and reported a result.
1 human study where nobody was given it
6 effects reported, 4 unwanted, 2 wanted, by people using it, not a trial
Goal pages: Fat loss and metabolism, Muscle and strength
In plain English
In plain English, from Aaron
This is how I would explain it to a friend. The evidence, with the numbers, is further down the page.
A big 55 mg vial with three peptides in it: 9 mg SS-31, 16 mg TB-500 and 30 mg MOTS-c. Do the division and the product flips. The one with a real drug trial behind it is the smallest part. The one with no human data is the biggest.
What people take it for
- Energy and stamina.
- Long term fatigue.
- Recovery from training.
What studies in people showed
Nobody has tested the three together. SS-31 is 16.4 percent of the powder. Its large final stage trial in a muscle disease of the cell's power plants did not show a real benefit in the group it studied. Some genetic subgroups did better on a walking test. MOTS-c is 54.5 percent of the powder and has no human trial at all. Its work is in fat mice. TB-500 is 29.1 percent and has no human study by any route. A drug testing lab found the product is a 7 amino acid piece of a 43 amino acid protein. And here is the odd bit. TB-500 is a healing peptide. It has nothing to do with mitochondria. No published paper explains why it is nearly a third of a mitochondria product. The math: mix 55 mg with 5 mL and every unit mark carries 60 micrograms of MOTS-c, 32 of TB-500 and 18 of SS-31.
What people report
Reports, not study results
The good
- More energy and better exercise tolerance in the first weeks.
- Better recovery from training, which people credit to the healing peptide rather than the mitochondria ones.
The bad
- Nothing at all.
- Sore shot sites.
- Complaints about cost, since the part people want is the smallest slice.
- One plunger moves all three, and the three do not share a schedule.
Where these come from: Peptide forums and seller product pages. People talking, using unverified material, usually alongside other things.
My bottom line
You are buying this for the SS-31 and getting mostly something else. When a vial gives you least of the thing with the best evidence, that tells you who the vial was designed for.
An opinion, not a finding. I am a coach, not a doctor.
What it has been measured to do
Measured in people
Goals Mitochondrial+ blend has been tested for in people, biggest studies first. We count only what the studies on this page say, so the real numbers may be higher.
- Getting strongerno headcount stated · 1 study
What people using it report
Real people telling you what happened to them. Nobody checked it, and the people who post are not a fair sample, so it cannot prove anything works. It is still the best guide on this page to what people actually do with Mitochondrial+ blend, what they expect, and what goes wrong. The full account, including the negative reports, is below.
Animal and lab work, not yet tested in people
Real results, but from animals or cells, not people. What works in a mouse often does not work the same way in a person. Nobody can patent this, so nobody would ever earn back the cost of a study in people. That is usually why there is none. The record on this one is set out below.
- Blood sugar and insulin1 animal or lab study
34 of the 36 indexed goals have no study of any kind behind Mitochondrial+ blend
No study in people, animals or cells on this page reports a result for these. That does not prove Mitochondrial+ blend does nothing for them. It does mean anything you have heard about Mitochondrial+ blend and one of these did not come from a study cited here.
Show full evidence
What is Mitochondrial+ blend?
Mitochondrial+ blend is a 55 mg vial of three peptides, sold at 9 mg SS-31, 16 mg TB-500 and 30 mg MOTS-c. MOTS-c is more than half the powder, and it has no study in people. SS-31, the one with a large final-stage study behind it, is the smallest share at 16.4%. The third is a repair peptide with no link to mitochondria at all.
In this vial, the math changes what the product is.
The split is 9 mg SS-31, 16 mg TB-500 and 30 mg MOTS-c, 55 mg of powder in all. That is MOTS-c at 54.5%, TB-500 at 29.1% and SS-31 at 16.4%. Mix it with 5 mL of water and each mL holds 11 mg of powder: 6 mg of MOTS-c, 3.2 mg of TB-500 and 1.8 mg of SS-31. One mark on a 100 unit insulin syringe gives 60 micrograms of MOTS-c, 32 micrograms of TB-500 and 18 micrograms of SS-31.
So the ingredient that finished a large final-stage drug programme is the one you get least of. The one with no study in people is the one you get most of. Whatever a buyer came for, by weight this is mostly a MOTS-c vial.
The third ingredient is the odd one. TB-500 is a repair peptide with no link to mitochondria. A drug testing lab for sport found it is a small piece of thymosin beta 4, just seven building blocks long, not the full protein with 43. It has no study in people by any route. No published reason explains why it is in a product sold for mitochondria. And it is nearly a third of the powder.
On the evidence. Elamipretide's large final-stage study in an inherited mitochondria muscle disease found no benefit across everyone. There were gains in some genetic groups, found by sorting the data after the fact. MOTS-c has mouse data only. TB-500 has none.
The timings also clash, in the usual way. People who use the TB-500 piece alone talk about milligram starting doses, then weekly doses. The two mitochondria peptides are each used on their own patterns. One plunger sets all three.
How does Mitochondrial+ blend work?
SS-31 sticks to a fat called cardiolipin in the inner wall of the mitochondria, and is said to steady it. MOTS-c is a peptide made from the mitochondria's own DNA, and is said to switch on AMPK, a fuel sensor in cells. The TB-500 piece sticks to actin, a building block inside cells, and has no known role in mitochondria. Two of the three aim at the mitochondria. The third does not, and no paper explains why it is there.
What have studies in people found about Mitochondrial+ blend?
The smallest ingredient by weight is the only one with a study in people, and that study failed its main test. The biggest ingredient has no data in people, and neither does the middle one.
- SS-31, 16.4% of the vial: its large final-stage study found no benefit across everyone. Some genetic groups did better, found by sorting the data after the fact.
- MOTS-c, 54.5% of the vial: no study in people. Only mouse blood marker work.
- TB-500, 29.1% of the vial: no study in people by any route. The product is a seven building block piece of a protein with 43.
- The blend: no published study and no listed trial.
What this does not tell you: Nobody knows what the three peptides do together. And nothing in the published record explains why a repair peptide makes up nearly a third of a mitochondria product.
What do people who took Mitochondrial+ blend report?
This is what real people say happened to them, good and bad. Nobody measured it. A report cannot tell a real effect from the lift people get just from expecting it to work, from a diet or training change made at the same time, or from what was really in the vial. We still put it near the top. It is the next best thing to a study in people: what happens when real people use it, including what goes wrong.
- More energy and handling exercise better in the first weeks.
- Better recovery from training. People credit the repair peptide for this, not the mitochondria ones.
- Nothing at all.
- Reactions where the shot goes in.
- Complaints about cost. The vial is big for a peptide, and the ingredient people want is the smallest share of it.
Sources: Peptide forums and vendor product pages. Uncontrolled self-reports from people using unverified material, frequently alongside other compounds.
The state of the research
The mix is the finding on this page. The vial is sold on the strength of a drug that reached a large final-stage study. But it gives you that drug as the smallest of three ingredients, and the untested one as the biggest. This pattern shows up again and again with blends. You only see it if someone does the division.
The repair peptide is the second thing. No published work links the TB-500 piece to mitochondria. And it has no study in people by any route. Adding it raises the weight, the price and the number of unknowns, without any reason anyone has written down.
The evidence, in charts
Fig. 1a · how many people
1study in people cited, but none says how many people took part
number of people not stated
We do not draw a total, because none of the studies in people on this page gives one. Read the sources below for what each study measured.
We read the number of people from the source lines on this page. Where a line gives no number, we count nothing for it. We do not guess.
Fig. 1b · evidence mix
1 of 4 sources here is a study in people. The rest are not.
- Study in people · done in people125%
- Animal study · done in animals125%
- Lab (cells in a dish) · cells or tissue125%
- Review of studies · sums up other studies125%
Counted from the list of sources on this page. It counts only what we cite, so there may be more studies out there. A review counts once, however many studies it sums up.
Fig. 2 · evidence over time
The studies cover 4 different years, 2012 to 2025, counted from the list of sources on this page.
Fig. 3 · legal status at a glance
US
Not approved
UK
Not approved
AU
Not approved
CA
Not approved
Not approved in any of the four countries shown. This is what the law says, not a verdict on the science. See Legal status below for the full text and any notes.
Fig. 4 · how much does what
Nobody has tested different amounts in people
This chart would show how the effect changes as the amount goes up. For this compound, nobody has measured that in people. So we leave it empty. We will not borrow an animal chart and pretend it works the same in people.
Fig. 5 · what the molecule is
- Kind of compound
- Blend
- Molecule weight
- A mixture. MOTS-c is a 16 amino acid peptide, SS-31 a tetrapeptide, and the fragment sold as TB-500 about 889 Da.
- Half-life
- Stated in words, not a numbersee the exact wording below
- Chain length
- None on file
Half-life as stated on file: Not defined for the blend. Three components with different clearance and no pharmacokinetic study of the mixture.
No amino acid sequence is on file for Mitochondrial+ blend, which is expected: a blend is not built from amino acids.
Fig. 6 · what is in the vial
54.5%of a 55 mg Mitochondrial+ blend vial is MOTS-c
- MOTS-c30 mg54.5%
- TB-50016 mg29.1%
- SS-31 (elamipretide)9 mg16.4%
What the label says is in a standard vial. It is not a dose, and not a lab test. The seller fixes the mix, so when you draw the amount you want of one ingredient, you get a set amount of every other one with it. No study has tested this mix in people or animals.
The studies we cite
- Study in people2024Genotype-specific effects of elamipretide in patients with primary mitochondrial myopathy: a post hoc analysis of the MMPOWER-3 trial
Elamipretide's main study did not work, and these numbers come from digging through the results afterwards. Across all the adults with an inherited mitochondria muscle disease, there was no clear benefit. Sorted by genetic type after the fact, one group walked about 25 metres further in six minutes than the dummy group. Another walked about 37 metres further. Hunting for winning groups once the data is already in hand is how false findings get made. None of this is proof until a new study tests it.
MMPOWER-3 as published did not demonstrate a significant benefit of elamipretide in a genotypically diverse adult population with primary mitochondrial myopathy. Post hoc, the mitochondrial DNA replisome cohort improved 25.2 metres on the six-minute walk against 2.0 metres on placebo at p = 0.06, and the chronic progressive external ophthalmoplegia subset improved 37.3 metres against a 8.0 metre decline at p = 0.0024.
- Review of studies2025Emerging Use of BPC-157 in Orthopaedic Sports Medicine: A Systematic Review
This review looked hard for BPC-157 studies and found almost none in people. Out of 36 studies it kept, 35 were in animals or in a dish. The one study in people looked back at 12 patients with knee pain who had the peptide put into the knee. 7 of the 12 said the pain eased for more than six months. There was no comparison group. The review says the peptide lasts under 30 minutes in the body.
544 articles screened, 36 included, of which 35 were preclinical and one was clinical. That single clinical study was a retrospective series in which 7 of 12 patients reported relief beyond six months after intra-articular BPC-157 for unspecified chronic knee pain. The review puts the half-life at under 30 minutes.
- Animal study2019The mitochondrial-derived peptide MOTS-c is a regulator of plasma metabolites and enhances insulin sensitivity
In mice made fat on a rich diet, MOTS-c lowered three kinds of fat and fuel breakdown products in the blood. These markers run high in fat and diabetic mice. The paper says the mice used insulin better and burned more fat. This was mice only. The lead scientist advises and owns shares in a mitochondria peptide company, and the paper says so.
In diet-induced obese mice, MOTS-c reduced sphingolipid, monoacylglycerol and dicarboxylate metabolism pathways, which are upregulated in obesity and diabetes models, with the paper describing improved insulin sensitivity and increased beta oxidation. Mice. The senior author discloses a consulting and stockholding interest in a mitochondrial peptide company.
- Lab (cells in a dish)2012Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential
This is a lab test of what is really in a TB-500 vial. A drug testing lab for sport ran the product through its machines. It was not the full thymosin beta 4 protein, which has 43 building blocks. It was a small piece of it, just seven building blocks long. The studies people quote for TB-500 were done on the full protein.
A doping control laboratory ran the TB-500 product through high performance liquid chromatography and high resolution mass spectrometry and identified the contents as Ac-LKKTETQ, the acetylated 17 to 23 fragment of thymosin beta 4. Seven amino acids, not the 43 amino acid protein the marketing names.
Where to buy Mitochondrial+ blend
1 seller lists Mitochondrial+ blend in our latest read. They are ranked by trust score, then by archive rating, then by certificate tier, then by the lowest price per milligram. Archive evidence is counted before source-backed. Each path is out of 4, and the two are not added. A higher price does not raise the score. The letter is the certificate chip. 0 of them have a published certificate that names Mitochondrial+ blend.
Some seller links may pay this site when you buy through them. A payment never changes a seller's tier or rank, and no seller has a say in any page.
| Seller | Trust score | Vial sizes | From, per mg | Certificate for Mitochondrial+ blend | Seller link |
|---|---|---|---|---|---|
| Puratek Peptides | 78 StrongSource-backed 4 of 4. Archive evidence 3 of 4.A | 120 mg | $0.625 | None found for Mitochondrial+ blend | View |
- LC Lab-confirmed: the issuing laboratory echoed this certificate back
- A Current certificate, independent laboratory named, laboratory not yet checked
- B Certificate published, but no laboratory link, older than a year, or the laboratory did not recognise the published lookup
- C No certificate could be read
Every listing and vial size for Mitochondrial+ blend · How the trust score is counted
Sellers named on the price pages are ranked by certificate tier, then price, and any affiliate income is disclosed there. Naming a seller is not a recommendation to buy or use anything.
Common questions
Has Mitochondrial+ blend been tested in people?
Not in a completed trial cited here. 1 human study where nobody was given it. Mitochondrial+ blend is a 55 mg vial of three peptides, sold at 9 mg SS-31, 16 mg TB-500 and 30 mg MOTS-c.
What is the Mitochondrial+ blend dosage?
No human dose established. No published study on this page has given this compound to people and reported a result. 1 human study where nobody was given it
Is Mitochondrial+ blend FDA-approved?
Not approved. None of the three parts is approved for sale, and no regulator has reviewed the combination.
What fraction of the vial is each component?
In the 55 mg vial, MOTS-c is 54.5%, TB-500 is 29.1% and SS-31 is 16.4%. Mixed with 5 mL of water, one unit holds 60 micrograms of MOTS-c, 32 micrograms of TB-500 and 18 micrograms of SS-31.
Why is a repair peptide in a mitochondrial blend?
No published reason explains it. The piece of protein sold as TB-500 has no known role in mitochondria and no study in people by any route. And it is nearly a third of the powder.
Which component has the best evidence?
SS-31, which is elamipretide. It is also the smallest share of the vial. Its large final-stage study found no benefit across everyone, with gains in some genetic groups found by sorting the data afterwards.
Has the three-way combination been studied?
No, in people or animals.
Can I adjust one component?
No. One plunger moves all three, and the timings they are each used on do not fit together.