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Safety / Published Sep 8, 2026 / Reviewed Sep 27, 2026 / 8 min read

How to Read a Peptide Certificate of Analysis: HPLC Purity, Mass Spec Identity, Endotoxin, and Janoshik Verification

Written by Reviewed Sep 27, 2026

What each line on a peptide lab certificate means, what a purity number does and does not tell you, how to check a Janoshik report, and the warning signs the FDA's own 2026 review found in products on the market. Plain English. No seller recommendations.

Key takeaways

  • A lab certificate, often called a COA (certificate of analysis), reports what a lab measured in one sample. It does not prove the product is safe. It only describes the vial in your hand if the batch number matches.
  • Four lines matter. Identity: is it the right molecule? Purity: how much of what the lab saw was the peptide? Amount: how many milligrams are really in there? Endotoxin: is there leftover germ material in it?
  • '99 percent pure' means 99 percent of what the lab's machine picked up was the peptide. It does not say how many milligrams are in the vial, whether the weight includes a salt, or whether the vial is sterile.
  • Janoshik reports carry a task number and a key you can check at verify.janoshik.com. Compare every line, not just the headline number.
  • The FDA's July 2026 review of BPC-157 products found certificates that did not match what the label said was inside, and labels that mixed up the pure peptide weight and the salt weight.

What is a certificate of analysis?

Direct answer

A certificate of analysis is a lab report on tests run on one sample. It usually covers identity, purity and amount, and sometimes germ material or sterility. It covers one batch only. It does not prove the product is safe, it does not cover how the vial was stored after testing, and it does not apply to any vial with a different batch number.

This guide is about reading the report, not about where to buy anything. The Longevity Archive names sellers only on its price pages, sorted by how well their certificates hold up, and this guide names none. The reason to understand a certificate is that the math on every dose depends on it. If a vial labeled 10 mg holds 7 mg, every number the calculator gives you is 30 percent off.

The four lines that matter

LineHow the lab tests itWhat it tells youWhat it does not tell you
IdentityMass spectrometry (LC-MS or MALDI), a machine that weighs moleculesThe molecule weighs what the peptide should weighWhether the building blocks are in the right order, if a look-alike weighs the same; whether it is the salt form on the label
PurityHPLC, a machine that separates what is in the sample and reads it with UV light, usually at 214 or 220 nmWhat share of everything the machine saw was the main peptideHow much peptide is in the vial; what the other things are; whether there is stuff the UV light can't see, like salts, water or germ material
AmountA measuring version of HPLC, or a count of the peptide's building blocks, checked against a known sampleHow many milligrams of peptide are really thereWhether the number is the pure peptide or includes the salt (an acetate salt can be 10 to 15 percent of the weight)
EndotoxinLAL test, reported in units (EU) per mg or per vialHow much leftover germ material there is. This is what causes redness at the shot spot and fever.Whether there are live germs, or heavy metals. Those need separate tests.
The most common gap is a certificate with purity but no amount. A vial can be 99 percent pure and half full. The amount is the number that decides your dose.

What is a good purity level for peptides?

Direct answer

Approved peptide drugs are usually held to above 98 or 99 percent purity, with named and limited leftovers. For research peptides, 98 percent or higher is the usual claim. But purity is not the whole story. A 99 percent pure vial holding 6 mg when the label says 10 mg, or one with lots of germ material, is worse than a 97 percent pure vial that is filled right and clean.

How do you verify a Janoshik report?

Direct answer

Every real Janoshik report has a task number and a key. Enter both at verify.janoshik.com. Then compare the compound name, batch, purity, identity and amount to the report you were given. A purity number alone does not prove the vial holds the labeled amount, holds the right molecule or is free of germ material.

  1. 01Find the task number and key on the report. They are printed on the report itself, not in the file name.
  2. 02Open the checking page and enter both. If the report is not there, it is not a Janoshik report.
  3. 03Check that the sample name, batch and date on the checked report match the copy you have, and match your vial.
  4. 04Compare every measured line, not just purity. Edited PDFs usually change one number and leave the rest.
  5. 05Note who sent the sample in. Janoshik tests what it is sent. It does not pick the vial or vouch for where it came from.

Is Janoshik legit? It is an independent lab in the Czech Republic. It publishes reports anyone can check, and that is why so many people use it. Whether the lab is real is not the question. The question is whether the sample it tested is the same as what you got, and no lab can answer that.

Red flags the FDA found

For its July 2026 advisory panel review of BPC-157, the FDA wrote a staff paper (FDA media 193343). It reported products on the market whose certificates did not match the named substance, and labels that mixed up the pure peptide weight and the salt weight. Those are two of the most common ways a certificate misleads:

  • Wrong molecule on the report. The certificate names a related compound, a piece of the peptide or a different salt from the label.
  • Salt weight or pure weight. A 10 mg acetate salt holds roughly 8.5 to 9 mg of actual peptide. Reports that don't say which one they measured leave a 10 to 15 percent gap.
  • Purity but no amount. Covered above.
  • No batch number, or a batch that does not match the vial.
  • A report older than the product's stated shelf life, or one dated before the batch was made.
  • 'Endotoxin: pass' with no number. A real LAL result is a number with units.

How do I know if my peptides are real?

Direct answer

You can't tell from the label. A certificate that matches your batch, with identity, amount and germ-material results you can check, is the best sign you can get. Sending your own vial to a lab is the only proof. Testing one sample costs roughly 60 to 200 US dollars, depending on the tests. That is why people pool money for 'group tests'.

You can't test at home with any accuracy. The machines that check identity and purity are lab machines. How clear it looks, its color and how it dissolves tell you almost nothing. But if you see bits floating in it, or it turns cloudy after mixing, don't use that vial (see the storage and mixing FAQ).

Why this matters more than the compound

Most compounds people test are unapproved. As our profiles show, most have few or no studies in people. The one thing a user can actually check is whether the vial holds what it says. That is a smaller question than 'does it work', and it is the only one a certificate can answer.

Frequently asked questions

What is Janoshik?

An independent lab in the Czech Republic. It tests peptides and other compounds to check what they are, how pure they are and how much is there. Anyone can check its reports using the task number.

How much does Janoshik cost?

The lab sets its prices, and they change. Testing one sample has usually cost somewhere from tens of dollars to a couple of hundred, depending on the tests. Check the lab's site for current prices.

Which peptide companies use Janoshik?

This site does not list or rate sellers here. Any seller can send in samples. What matters is whether the report you are shown can be checked and matches your batch.

Can a COA be faked?

PDFs can be edited. That is why checking the task number on the lab's own site matters, and why every line should match, not just purity.

Compound profiles mentioned

Sources

  • Review of studies2026

    FDA briefing document: BPC-157 (PCAC July 2026)

    Certificates that did not match the labeled substance; labels that mixed up pure peptide weight and salt weight.

    FDA
  • Review of studies2026

    Janoshik Analytical: report verification

    Reports can be checked with a task number and key.

    Janoshik
  • Review of studies2021

    Characterization of Synthetic Peptide Therapeutics Using Liquid Chromatography-Mass Spectrometry: Challenges, Solutions, Pitfalls, and Future Perspectives

    The standard methods for identity and purity of a synthetic peptide, what each one answers, and the pitfalls. Verified on PubMed 1 October 2026.

    Journal of the American Society for Mass Spectrometry
  • Review of studies2020

    Currently Available Recombinant Alternatives to Horseshoe Crab Blood Lysates: Are They Comparable for the Detection of Environmental Bacterial Endotoxins? A Review

    How the bacterial endotoxins test works and reports, and how the recombinant alternatives compare with horseshoe crab lysate. Verified on PubMed 1 October 2026.

    PDA Journal of Pharmaceutical Science and Technology

Read next

Educational use only. This database summarizes published research and is not medical advice. Where a dose appears it is a record of what a trial or clinic ran, or what people describe doing, with its source attached. Nothing here is a recommendation for personal use. Sellers named on the price pages are ranked by certificate tier, then price, and any affiliate income is disclosed there. Naming a seller is not a recommendation to buy or use anything.